Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of NIH/3T3 using 10518-2-AP
WB result of PACSIN2 antibody (10518-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACSIN2 transfected NIH/3T3 cells.
WB analysis using 10518-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10518-2-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10518-2-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 10518-2-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 10518-2-AP
IP result of anti-PACSIN2 (IP:10518-2-AP, 4ug; Detection:10518-2-AP 1:500) with NIH/3T3 cells lysate 1200ug.
IHC staining of human breast cancer using 10518-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human breast cancer using 10518-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human skin using 10518-2-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human skin using 10518-2-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).
IF Staining of NIH/3T3 using 10518-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PACSIN2 antibody (10518-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

PACSIN2 Polyclonal antibody

PACSIN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10518-2-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Syndapin-II, Syndapin-2, SdpII, Protein kinase C and casein kinase substrate in neurons protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse heart tissue, mouse lung tissue, NIH/3T3 cells, HepG2 cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IF	See 5 publications below

Product Information

10518-2-AP targets PACSIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0809 Product name: Recombinant human PACSIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-486 aa of BC008037 Sequence: PSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ 相同性解析による交差性が予測される生物種
Full Name	protein kinase C and casein kinase substrate in neurons 2
Calculated molecular weight	56 kDa
Observed molecular weight	60-65 kDa
GenBank accession number	BC008037
Gene Symbol	PACSIN2
Gene ID (NCBI)	11252
RRID	AB_2161854
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNF0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

Protocols

Product Specific Protocols
IF protocol for PACSIN2 antibody 10518-2-AP	Download protocol
IHC protocol for PACSIN2 antibody 10518-2-AP	Download protocol
IP protocol for PACSIN2 antibody 10518-2-AP	Download protocol
WB protocol for PACSIN2 antibody 10518-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Biol Cell Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis. Authors - Giridharan Sai Srinivas Panapakkam SS KD Validated View Article
human	WB	PLoS One Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization. Authors - Simone Laura C LC KD Validated View Article
human	WB,IF	J Bacteriol Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells. Authors - Izumi Yanatori View Article
human	WB	Cell Rep Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation. Authors - Ke Chen View Article
human,mouse,zebrafish	WB,IF	Nat Commun Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Authors - Christine Insinna KO Validated View Article
human	WB	J Dent Res Proteome Analysis of Temporomandibular Joint with Disc Displacement Authors - X Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of NIH/3T3 using 10518-2-AP

WB analysis using 10518-2-AP

WB analysis of NIH/3T3 using 10518-2-AP

WB analysis of mouse lung using 10518-2-AP

WB analysis of mouse heart using 10518-2-AP

IP experiment of NIH/3T3 using 10518-2-AP

IHC staining of human breast cancer using 10518-2-AP

IHC staining of human breast cancer using 10518-2-AP

IHC staining of human skin using 10518-2-AP

IHC staining of human skin using 10518-2-AP

IF Staining of NIH/3T3 using 10518-2-AP

PACSIN2 Polyclonal antibody

PACSIN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.

Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization.

Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells.

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.

Proteome Analysis of Temporomandibular Joint with Disc Displacement