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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PACS2 Polyclonal antibody (30172-1-AP)

    WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    IP experiment of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using PACS2 Polyclonal antibody (30172-1-AP)

    IF Staining of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PACS2 Polyclonal antibody, PBS Only

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 30172-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30172-1-PBS targets PACS2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32902

Product name: Recombinant human PACS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 340-500 aa of NM_001100913

Sequence: SARSHKEPPSPADVPEKTRSLGGRQPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDVFTERLPPSGRITKTESLVIPSTRSEGKQAGRRGRSTSLKERQAARPQNERANSLDNERCPDARSQLQIPRKTVYDQLNHILISDDQLPENIILVNTSDWQ

相同性解析による交差性が予測される生物種
Full Name phosphofurin acidic cluster sorting protein 2
Calculated molecular weight98kd
Observed molecular weight100-130 kDa
GenBank accession numberNM_001100913
Gene Symbol PACS2
Gene ID (NCBI) 23241
RRIDAB_3086254
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VP3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

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