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PABPC4 Polyclonal antibody
PABPC4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14960-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse heart tissue, human brain tissue, Jurkat cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
14960-1-AP targets PABPC4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PABPC4 fusion protein Ag6813 相同性解析による交差性が予測される生物種 |
Full Name | poly(A) binding protein, cytoplasmic 4 (inducible form) |
Calculated molecular weight | 70 kDa |
Observed molecular weight | 70-71 kDa |
GenBank accession number | BC065540 |
Gene symbol | PABPC4 |
Gene ID (NCBI) | 8761 |
RRID | AB_2156897 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PABPC4 antibody 14960-1-AP | Download protocol |
IHC protocol for PABPC4 antibody 14960-1-AP | Download protocol |
IF protocol for PABPC4 antibody 14960-1-AP | Download protocol |
IP protocol for PABPC4 antibody 14960-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. | ||
J Immunol HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
| ||
BMC Pregnancy Childbirth Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics. | ||
PLoS Pathog Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation | ||
Nat Cell Biol An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus |