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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

    WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    WB analysis of LNCaP using 66809-1-Ig (same clone as 66809-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IHC staining of mouse cerebellum using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PABPC1,PABP Monoclonal antibody (66809-1-Ig)

    IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PABPC1,PABP Monoclonal antibody, PBS Only

PABPC1,PABP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66809-1-PBS
Clone No.3D8B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PABP, PABP1, PABPC1, 3D8B5, PABP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66809-1-PBS targets PABPC1,PABP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27860

Product name: Recombinant human PABPC1,PABP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 287-636 aa of BC015958

Sequence: RITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV

相同性解析による交差性が予測される生物種
Full Name poly(A) binding protein, cytoplasmic 1
Calculated molecular weight 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC015958
Gene Symbol PABPC1
Gene ID (NCBI) 26986
RRIDAB_2882152
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11940
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].