Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 17650-1-AP (same clone as 17650-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 17650-1-AP (same clone as 17650-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 17650-1-AP (same clone as 17650-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

PAAF1 Polyclonal antibody, PBS Only

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17650-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17650-1-PBS targets PAAF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11892 Product name: Recombinant human PAAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC028628 Sequence: MLVPCFLYSLQNRKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL 相同性解析による交差性が予測される生物種
Full Name	proteasomal ATPase-associated factor 1
Calculated molecular weight	375 aa, 40 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC028628
Gene Symbol	PAAF1
Gene ID (NCBI)	80227
RRID	AB_2878424
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BRP4
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)

WB analysis of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

WB analysis of human brain using 17650-1-AP (same clone as 17650-1-PBS)

WB analysis of HEK-293 using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)