Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 14632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 14632-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 14632-1-AP
IP result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of human breast cancer using 14632-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14632-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14632-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of hTERT-RPE1 using 14632-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14632-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DDX6 Polyclonal antibody

DDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14632-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ATP-dependent RNA helicase p54, EC:3.6.4.13, HLR2, P54, Probable ATP-dependent RNA helicase DDX6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, C2C12 cells, mouse testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human breast cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IF	See 7 publications below

Product Information

14632-1-AP targets DDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6211 Product name: Recombinant human P54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-483 aa of BC065007 Sequence: RDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP 相同性解析による交差性が予測される生物種
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Calculated molecular weight	54 kDa
Observed molecular weight	54 kDa
GenBank accession number	BC065007
Gene Symbol	DDX6
Gene ID (NCBI)	1656
RRID	AB_2091264
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26196
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Protocols

Product Specific Protocols
IF protocol for DDX6 antibody 14632-1-AP	Download protocol
IHC protocol for DDX6 antibody 14632-1-AP	Download protocol
IP protocol for DDX6 antibody 14632-1-AP	Download protocol
WB protocol for DDX6 antibody 14632-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB,IF	Adv Sci (Weinh) Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia Authors - Ningjing Ou View Article
mouse	WB,IF	J Genet Genomics LSM14B coordinates protein component expression in the P-body and controls oocyte maturation Authors - Huiru Zhang View Article
human	WB,IF	iScience The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex. Authors - Limei Zou View Article
mouse	WB	Biol Reprod Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development. Authors - Chappell Vesna A VA View Article
human	WB	Biochem Biophys Res Commun DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation Authors - Seiichiro Naito KD Validated View Article
human	WB	EMBO J Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1 Authors - Wanqi Zhan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14632-1-AP

WB analysis using 14632-1-AP

WB analysis using 14632-1-AP

IP experiment of mouse testis using 14632-1-AP

IHC staining of human breast cancer using 14632-1-AP

IHC staining of mouse testis using 14632-1-AP

IHC staining of mouse testis using 14632-1-AP

IHC staining of human breast cancer using 14632-1-AP

IF Staining of hTERT-RPE1 using 14632-1-AP

IF Staining of HeLa using 14632-1-AP

DDX6 Polyclonal antibody

DDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.

Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.

DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation

Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1