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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P4HA3 Recombinant monoclonal antibody (85374-1-RR)

    WB analysis using 85374-1-RR (same clone as 85374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85374-1-RR (P4HA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using P4HA3 Recombinant monoclonal antibody (85374-1-RR)

    IHC staining of human stomach cancer using 85374-1-RR (same clone as 85374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85374-1-RR (P4HA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using P4HA3 Recombinant monoclonal antibody (85374-1-RR)

    IHC staining of human stomach cancer using 85374-1-RR (same clone as 85374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85374-1-RR (P4HA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using P4HA3 Recombinant monoclonal antibody (85374-1-RR)

    IF Staining of A549 using 85374-1-RR (same clone as 85374-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using P4HA3 antibody (85374-1-RR, Clone: 242750C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • Affinity of 85374-1-RR

    Affinity of 85374-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85374-1-RR against Human P4HA3 were performed. The affinity constant is below 1 pM.

P4HA3 Recombinant monoclonal antibody, PBS Only

P4HA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85374-1-PBS
Clone No.242750C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

4-PH alpha-3, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85374-1-PBS targets P4HA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19716

Product name: Recombinant human P4HA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-256 aa of BC126170

Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY

相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, alpha polypeptide III
Calculated molecular weight 544 aa, 61 kDa
Observed molecular weight61 kDa
GenBank accession numberBC126170
Gene Symbol P4HA3
Gene ID (NCBI) 283208
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7Z4N8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

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