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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using P4HA2 Monoclonal antibody (66604-1-Ig)

    WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using P4HA2 Monoclonal antibody (66604-1-Ig)

    WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using P4HA2 Monoclonal antibody (66604-1-Ig)

    WB analysis of NIH/3T3 using 66604-1-Ig (same clone as 66604-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using P4HA2 Monoclonal antibody (66604-1-Ig)

    WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P4HA2 Monoclonal antibody (66604-1-Ig)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P4HA2 Monoclonal antibody (66604-1-Ig)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

P4HA2 Monoclonal antibody, PBS Only

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66604-1-PBS
Clone No.3A9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66604-1-PBS targets P4HA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4714

Product name: Recombinant human P4HA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 184-533 aa of BC035813

Sequence: HTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRRPFDSGLKTEGNRLATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD

相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, alpha polypeptide II
Calculated molecular weight 533 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC035813
Gene Symbol P4HA2
Gene ID (NCBI) 8974
RRIDAB_2881964
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO15460
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

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