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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of HEK-293 using 66101-1-Ig (same clone as 66101-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of Neuro-2a using 66101-1-Ig (same clone as 66101-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of HSC-T6 using 66101-1-Ig (same clone as 66101-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of ROS1728 using 66101-1-Ig (same clone as 66101-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of 4T1 using 66101-1-Ig (same clone as 66101-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of rat liver using 66101-1-Ig (same clone as 66101-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using P4HA1 Monoclonal antibody (66101-1-Ig)

    WB analysis of NIH/3T3 using 66101-1-Ig (same clone as 66101-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using P4HA1 Monoclonal antibody (66101-1-Ig)

    IHC staining of human testis using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

P4HA1 Monoclonal antibody, PBS Only

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66101-1-PBS
Clone No.1H10A6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1H10A6, EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66101-1-PBS targets P4HA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P4HA1 fusion protein Ag17867 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, alpha polypeptide I
Calculated molecular weight 534 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC034998
Gene Symbol P4HA1
Gene ID (NCBI) 5033
RRIDAB_2881500
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13674
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.