Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15723-1-AP (same clone as 15723-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
WB analysis of mouse placenta using 15723-1-AP (same clone as 15723-1-PBS)
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 15723-1-AP (same clone as 15723-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 15723-1-AP (same clone as 15723-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)
IP result of anti-P3H2 (IP:15723-1-AP, 4ug; Detection:15723-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 15723-1-AP (same clone as 15723-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15723-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

P3H2 Polyclonal antibody, PBS Only

P3H2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15723-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15723-1-PBS targets P3H2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8444 Product name: Recombinant human LEPREL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 179-527 aa of BC005029 Sequence: ASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL 相同性解析による交差性が予測される生物種
Full Name	leprecan-like 1
Calculated molecular weight	708aa,81 kDa; 527aa,60 kDa
Observed molecular weight	80 kDa
GenBank accession number	BC005029
Gene Symbol	LEPREL1
Gene ID (NCBI)	55214
RRID	AB_2136486
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IVL5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15723-1-AP (same clone as 15723-1-PBS)

WB analysis of mouse placenta using 15723-1-AP (same clone as 15723-1-PBS)

WB analysis of HEK-293 using 15723-1-AP (same clone as 15723-1-PBS)

WB analysis of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)

WB analysis of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

IP experiment of mouse kidney using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human liver using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human placenta using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human testis using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human skin using 15723-1-AP (same clone as 15723-1-PBS)

IHC staining of human brain using 15723-1-AP (same clone as 15723-1-PBS)