Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spinal cord tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 1 publications below |
Product Information
11976-1-AP targets P2RY12 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2601 Product name: Recombinant human P2RY12 protein Source: e coli.-derived, T-GST Tag: GST Domain: 1-342 aa of BC017898 Sequence: MQAVDNLTSAPGNTSLCTRDYKITQVLFPLLYTVLFFVGLITNGLAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDAKLGTGPLRTFVCQVTSVIFYFTMYISISFLGLITIDRYQKTTRPFKTSNPKNLLGAKILSVVIWAFMFLLSLPNMILTNRQPRDKNVKKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYTLITKELYRSYVRTRGVGKVPRKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLISMLKCPNSATSLSQDNRKKEQDGGDPNEETPM 相同性解析による交差性が予測される生物種 |
| Full Name | purinergic receptor P2Y, G-protein coupled, 12 |
| Calculated molecular weight | 342 aa, 39 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC017898 |
| Gene Symbol | P2RY12 |
| Gene ID (NCBI) | 64805 |
| RRID | AB_11182489 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H244 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
P2RY12 is a Gi-coupled receptor containing 342 amino acids and with a molecular weight of 39 kilodaltons. Initially, P2RY12 was identified on platelets and in a lesser quantity in some regions of the brain. However, it is now well established that P2RY12 has a wider cell expression, as it is also expressed on vascular smooth muscle cells, brown adipocytes, cholangiocyte primary cilia, osteoblasts, osteoclasts, and several immune cells including dendritic cells and lymphocytes. (PMID: 33561958)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY12 antibody 11976-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Xuefu Zhuyu Decoction Ameliorates Neuroinflammation after Traumatic Brain Injury via tRF-3-Leu-AAG/Gpr17 axis. | ||
Cell Rep Microglial macrophage-derived ds-HMGB1 in DRG orchestrates neuropathic pain through immune-neural signaling. | ||
Cell Mol Life Sci Multiplexed mRNA analysis of brain-derived extracellular vesicles upon experimental stroke in mice reveals increased mRNA content with potential relevance to inflammation and recovery processes. | ||
J Am Heart Assoc Influence of GAS5/MicroRNA-223-3p/P2Y12 Axis on Clopidogrel Response in Coronary Artery Disease | ||
Front Pharmacol Transdermal administration of herbal essential oil alleviates high-fat diet-induced obesity by regulating metabolism and gut microbiota |