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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using P2RY1 Polyclonal antibody (18273-1-AP)

    WB analysis of Jurkat using 18273-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18273-1-AP (P2RY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    WB analysis of mouse heart using 18273-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18273-1-AP (P2RY1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human kidney using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human kidney using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human placenta using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human placenta using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human lung using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human lung using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human ovary using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human ovary using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

P2RY1 Polyclonal antibody

P2RY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18273-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18273-1-AP targets P2RY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12992

Product name: Recombinant human P2RY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-373 aa of BC074785

Sequence: TMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSL

相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2Y, G-protein coupled, 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 42 kDa, 57 kDa-59 kDa, 66 kDa
GenBank accession numberBC074785
Gene Symbol P2RY1
Gene ID (NCBI) 5028
RRIDAB_2158402
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP47900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for P2RY1 antibody 18273-1-APDownload protocol
WB protocol for P2RY1 antibody 18273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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