• Featured Product
  • KD/KO Validated

P21 Monoclonal antibody

P21 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

monkey and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5F2F3

Cat no : 60214-1-Ig

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60214-1-Ig is P21 Fusion Protein expressed in E. coli.

Tested Reactivity monkey
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P21 fusion protein Ag0308 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight 21 kDa
GenBank accession numberBC000312
Gene symbol CDKN1A
Gene ID (NCBI) 1026
RRIDAB_11042450
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Protocols

Product Specific Protocols
WB protocol for P21 antibody 60214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.

Authors - Xiangdong Bai
humanWB

J Pathol

NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.

Authors - Rong-Zong Liu
  • KD Validated
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
WB

Aging (Albany NY)

Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.

Authors - Rika Imai
humanWB

J Biol Chem

RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Authors - Xin Lin
humanWB

Front Pharmacol

Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening

Authors - Wei Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (02-02-2021)

it is very good antibody.

  • Applications: Western Blot
FH

Chunxia (Verified Customer) (02-02-2021)

Tissue culture

  • Applications: Western Blot, Cell culture