Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, rat kidney tissue, human kidney tissue, LPS and Brefeldin A treated RAW 264.7 |
| Positive IF-P detected in | mouse stomach tissue |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 37 publications below |
| IHC | See 9 publications below |
| IF | See 19 publications below |
Product Information
30200-1-AP targets Osteopontin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg0317 Product name: Recombinant Mouse Osteopontin protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 17-294 aa of NM_001204233.1 Sequence: LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVN 相同性解析による交差性が予測される生物種 |
| Full Name | secreted phosphoprotein 1 |
| Calculated molecular weight | 32kd |
| Observed molecular weight | 32 kDa, 60-70 kDa |
| GenBank accession number | NM_001204233.1 |
| Gene Symbol | Osteopontin |
| Gene ID (NCBI) | 20750 |
| RRID | AB_3086261 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Osteopontin antibody 30200-1-AP | Download protocol |
| WB protocol for Osteopontin antibody 30200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration. | ||
Bioact Mater Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications. | ||
ACS Nano A Straw-Reinforced-Clay-Inspired Composite Hydrogel Promotes Diabetic Bone Regeneration via Driving the Immune-Osteogenic Cascade to Remodel Mitochondrial Homeostasis. | ||
J Nanobiotechnology Piezoelectric heterojunction/polyetherketoneketone composite with enhanced sonodynamic efficacy and nanozyme activities for anti-infection and encouraging neurovascular ossification. | ||
J Nanobiotechnology Functional-responsive hydrogel microspheres based on microenvironment sensing promote the sequential repair of infected bone defects by regulating ROS balance. | ||
Adv Sci (Weinh) Virulence-Selective Trap-Capture-Kill Antibacterial Nanostructures With Immune-Metabolic Regulation for Treating Implant-Associated Infections. |