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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Osteoglycin Polyclonal antibody (12755-1-AP)

    WB analysis of NIH/3T3 using 12755-1-AP (same clone as 12755-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Osteoglycin Polyclonal antibody (12755-1-AP)

    WB analysis of human liver using 12755-1-AP (same clone as 12755-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Osteoglycin Polyclonal antibody (12755-1-AP)

    WB analysis of HeLa using 12755-1-AP (same clone as 12755-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Osteoglycin Polyclonal antibody (12755-1-AP)

    IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Osteoglycin Polyclonal antibody (12755-1-AP)

    IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Osteoglycin Polyclonal antibody (12755-1-AP)

    IF Staining of HepG2 using 12755-1-AP (same clone as 12755-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoglycin antibody (12755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

Osteoglycin Polyclonal antibody, PBS Only

Osteoglycin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12755-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12755-1-PBS targets Osteoglycin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3484

Product name: Recombinant human OGN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC037273

Sequence: MKTLQSTLLLLLLVPLIKPAPPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF

相同性解析による交差性が予測される生物種
Full Name osteoglycin
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC037273
Gene Symbol OGN
Gene ID (NCBI) 4969
RRIDAB_10646479
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP20774
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

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