Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, PNGF treated mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32498-1-AP targets ORM1 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg2752 Product name: Recombinant Mouse ORM1 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-207 aa of NM_008768.2 Sequence: QNPEHANFTIGEPITNETLSWLSDKWFFMGAAFRKLEYRQAIQTMQSEFFYLTTNLINDTIELRESQTIGDQCVYNSTHLGFQRENGTFSKYEGGVETFAHLIVLRKHGAFMLAFDLKDEKKRGLSLYAKRPDITPELREVFQKAVTHVGMDESEIIFVDWKKDRCGQQEKKQLELGKETKKDPEEGQA 相同性解析による交差性が予測される生物種 |
| Full Name | orosomucoid 1 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 23-44 kDa |
| GenBank accession number | NM_008768.2 |
| Gene Symbol | Orm1 |
| Gene ID (NCBI) | 18405 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q60590 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Orosomucoid-1 (ORM1), also called Alpha-1-acid glycoprotein 1 (AGP1), is a glycoprotein synthesized mostly by hepatocytes. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in mouse kidneys which is due to the glycosylation of ORM1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ORM1 antibody 32498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |