Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroIF/ICCFC (Intra)

WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis using 27260-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IP experiment of HUVEC using 27260-1-AP
IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:2000) with HUVEC cells lysate 840 ug.
IHC staining of mouse colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 27260-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse colon using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Caco-2 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
IF Staining of hTERT-RPE1 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (27260-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
IF Staining of Caco-2 using 27260-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).
FC experiment of MCF-7 using 27260-1-AP
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Occludin Polyclonal antibody (27260-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Cat No. 27260-1-AP

Publications(1342)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Synonyms

OCLN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1342)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HUVEC cells, A431 cells, mouse kidney tissue, Caco-2 cells, mouse brain tissue, mouse colon tissue, rat colon tissue, rat brain tissue
Positive IP detected in	HUVEC cells
Positive IHC detected in	mouse colon tissue, human colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	Caco-2 cells, MCF-7 cells, hTERT-RPE1 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:400-1:2000
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 896 publications below
IHC	See 195 publications below
IF	See 415 publications below
IP	See 1 publications below

Product Information

27260-1-AP targets Occludin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, chicken, sheep, goat, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26173 Product name: Recombinant human Occludin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-522 aa of BC029886 Sequence: VDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT 相同性解析による交差性が予測される生物種
Full Name	occludin
Calculated molecular weight	522 aa, 59 kDa
Observed molecular weight	59 kDa, 53 kDa, 32 kDa, 23-25 kDa
GenBank accession number	BC029886
Gene Symbol	Occludin
Gene ID (NCBI)	4950
RRID	AB_2880820
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16625
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini, and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
FC protocol for Occludin antibody 27260-1-AP	Download protocol
IF protocol for Occludin antibody 27260-1-AP	Download protocol
IHC protocol for Occludin antibody 27260-1-AP	Download protocol
IP protocol for Occludin antibody 27260-1-AP	Download protocol
WB protocol for Occludin antibody 27260-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Spermine is an endogenous iron chelator that inhibits ferroptosis Authors - Man Li View Article
mouse	WB,IF	Circulation Microplastic Exposure Aggravates Cardiomyopathy Under Hemodynamic Stress Through the Gut-Heart Axis Authors - Jing Wang View Article
	WB	Cell Metab Ketogenic diet alleviates septic lung injury via microbial gut-lung axis Authors - Mingyuan Wei View Article
mouse	IHC	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	WB	Exploration (Beijing) Ganoderic Acids Alleviate Neuroinflammation by Targeting Myeloid Differentiation Factor 2 for Ischemic Stroke Therapy. Authors - Ang Ma View Article
	IF	Gastroenterology Structural Variations in Ulcerative Colitis-associated Escherichia coli Reduce Fructose Utilization and Aggravate Inflammation Under High-Fructose Diet Authors - Xia Wu View Article

Validation Data Gallery

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

WB analysis using 27260-1-AP

IP experiment of HUVEC using 27260-1-AP

IHC staining of mouse colon using 27260-1-AP

IHC staining of mouse colon using 27260-1-AP

IHC staining of human colon using 27260-1-AP

IHC staining of human colon using 27260-1-AP

IHC staining of mouse colon using 27260-1-AP

IHC staining of rat colon using 27260-1-AP

IF Staining of mouse colon using 27260-1-AP

IF Staining of mouse brain using 27260-1-AP

IF Staining of Caco-2 using 27260-1-AP

IF Staining of hTERT-RPE1 using 27260-1-AP

IF Staining of MCF-7 using 27260-1-AP

IF Staining of Caco-2 using 27260-1-AP

FC experiment of MCF-7 using 27260-1-AP

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spermine is an endogenous iron chelator that inhibits ferroptosis

Microplastic Exposure Aggravates Cardiomyopathy Under Hemodynamic Stress Through the Gut-Heart Axis

Ketogenic diet alleviates septic lung injury via microbial gut-lung axis

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Ganoderic Acids Alleviate Neuroinflammation by Targeting Myeloid Differentiation Factor 2 for Ischemic Stroke Therapy.

Structural Variations in Ulcerative Colitis-associated Escherichia coli Reduce Fructose Utilization and Aggravate Inflammation Under High-Fructose Diet

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