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Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of Jurkat using 67836-1-Ig (same clone as 67836-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of pig brain using 67836-1-Ig (same clone as 67836-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of mouse brain using 67836-1-Ig (same clone as 67836-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    WB analysis of rabbit brain using 67836-1-Ig (same clone as 67836-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using OXCT1 Monoclonal antibody (67836-1-Ig)

    IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using OXCT1 Monoclonal antibody (67836-1-Ig)

    FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

OXCT1 Monoclonal antibody, PBS Only

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67836-1-PBS
Clone No.1G1B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1G1B9, 3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, EC:2.8.3.5, OXCT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67836-1-PBS targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24792

Product name: Recombinant human SCOT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 151-520 aa of BC009001

Sequence: GVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN

相同性解析による交差性が予測される生物種
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918598
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP55809
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

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