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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using OX40L/TNFSF4 Recombinant monoclonal antibody (82794-7-RR)

    WB analysis of HUVEC using 82794-7-RR (same clone as 82794-7-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using OX40L/TNFSF4 Recombinant monoclonal antibody (82794-7-RR)

    IHC staining of human tonsillitis using 82794-7-RR (same clone as 82794-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MJ cells using OX40L/TNFSF4 Recombinant monoclonal antibody (82794-7-RR)

    IF Staining of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MJ cells using OX40L/TNFSF4 Recombinant monoclonal antibody (82794-7-RR)

    FC experiment of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82794-7-RR

    Affinity and Kinetic Characterization of 82794-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.

OX40L/TNFSF4 Recombinant monoclonal antibody, PBS Only

OX40L/TNFSF4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA
Cat No. 82794-7-PBS
Clone No.241813A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC, Indirect ELISA

Synonyms

OX40L, TNFSF4, TNFSF4/CD252, 241813A6, OX 40L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82794-7-PBS targets OX40L/TNFSF4 in WB, IHC, IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0582

Product name: Recombinant Human OX40L/TNFSF4 protein (His Tag)

Source: mammalian cells-derived, pCDNA3.4-IGSF8(C6)

Tag: N-6*His

Domain: 51-183 aa of NM_003326.5

Sequence: QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 4
Observed molecular weight30 kDa
GenBank accession numberNM_003326.5
Gene Symbol OX40L/TNFSF4
Gene ID (NCBI) 7292
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23510
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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