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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OVGP1 Polyclonal antibody (22324-1-AP)

    WB analysis using 22324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22324-1-AP (OVGP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OVGP1 Polyclonal antibody (22324-1-AP)

    IHC staining of mouse ovary using 22324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22324-1-AP (OVGP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OVGP1 Polyclonal antibody

OVGP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22324-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CHIT5, EGP, MUC9, Mucin 9, OGP, OVGP1, Oviduct specific glycoprotein, Oviductal glycoprotein, Oviductin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse uterus tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22324-1-AP targets OVGP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17771

Product name: Recombinant human OVGP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 329-678 aa of BC126177

Sequence: VGYDNAISFSYKAWFIRREHFGGAMVWTLDMDDVRGTFCGTGPFPLVYVLNDILVRAEFSSTSLPQFWLSSAVNSSSTDPERLAVTTAWTTDSKILPPGGEAGVTEIHGKCENMTITPRGTTVTPTKETVSLGKHTVALGEKTEITGAMTMTSVGHQSMTPGEKALTPVGHQSVTTGQKTLTSVGYQSVTPGEKTLTPVGHQSVTPVSHQSVSPGGTTMTPVHFQTETLRQNTVAPRRKAVAREKVTVPSRNISVTPEGQTMPLRGENLTSEVGTHPRMGNLGLQMEAENRMMLSSSPVIQLPEQTPLAFDNRFVPIYGNHSSVNSVTPQTSPLSLKKEIPENSAVDEEA

相同性解析による交差性が予測される生物種
Full Name oviductal glycoprotein 1, 120kDa
Calculated molecular weight 678 aa, 75 kDa
Observed molecular weight70 kDa
GenBank accession numberBC126177
Gene Symbol OVGP1
Gene ID (NCBI) 5016
RRIDAB_3085696
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ12889
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OVGP1 (oviduct-specific glycoprotein, also known as MUC9) is a high molecular-weight glycoprotein thought to be exclusively secreted by the non-ciliated epithelial cells of the fallopian tube (PMID: 12444068). Identified first as an estrogen-induced secretory protein in the oviduct, OVGP1 aids in sperm capacitation, fertilization, and early embryonic development (PMID: 28883914). OVGP1 is the major non-serum protein present in OF (oviduct fluid) and its biological activity differs among species (PMID: 27601270).

Protocols

Product Specific Protocols
WB protocol for OVGP1 antibody 22324-1-APDownload protocol
IHC protocol for OVGP1 antibody 22324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols