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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OVCA2 Polyclonal antibody

OVCA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14943-1-AP

Synonyms

OVCA2

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 14943-1-AP

    WB analysis of human kidney using 14943-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 14943-1-AP

    WB analysis of mouse thymus using 14943-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 14943-1-AP

    WB analysis of human brain using 14943-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14943-1-AP

    IHC staining of mouse kidney using 14943-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14943-1-AP

    IHC staining of mouse kidney using 14943-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman kidney tissue, human brain tissue, mouse thymus tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14943-1-AP targets OVCA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OVCA2 fusion protein Ag6744 相同性解析による交差性が予測される生物種
Full Name candidate tumor suppressor in ovarian cancer 2
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC041170
Gene symbol OVCA2
Gene ID (NCBI) 124641
RRIDAB_2157286
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OVCA2 antibody 14943-1-APDownload protocol
IHC protocol for OVCA2 antibody 14943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols