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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTX2 Polyclonal antibody (13497-1-AP)

    WB analysis using 13497-1-AP (same clone as 13497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Y79 cells using OTX2 Polyclonal antibody (13497-1-AP)

    IP experiment of Y79 using 13497-1-AP (same clone as 13497-1-PBS)

    IP result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug. This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Y79 cells using OTX2 Polyclonal antibody (13497-1-AP)

    IP experiment of Y79 using 13497-1-AP (same clone as 13497-1-PBS)

    IP result of anti-OTX2 (IP:13497-1-AP, 4ug; Detection:13497-1-AP 1:2000) with Y79 cells lysate 2000ug. This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using OTX2 Polyclonal antibody (13497-1-AP)

    IHC staining of human gliomas using 13497-1-AP (same clone as 13497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using OTX2 Polyclonal antibody (13497-1-AP)

    IHC staining of human gliomas using 13497-1-AP (same clone as 13497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using OTX2 Polyclonal antibody (13497-1-AP)

    FC experiment of HepG2 using 13497-1-AP (same clone as 13497-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

OTX2 Polyclonal antibody, PBS Only

OTX2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 13497-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

MCOPS5, Homeobox protein OTX2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13497-1-PBS targets OTX2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTX2 fusion protein Ag4323 相同性解析による交差性が予測される生物種
Full Name orthodenticle homeobox 2
Calculated molecular weight 297 aa, 32 kDa
Observed molecular weight 31-35 kDa
GenBank accession numberBC032579
Gene Symbol OTX2
Gene ID (NCBI) 5015
RRIDAB_2157176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32243
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive