Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26595-1-AP targets OTX1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24252 Product name: Recombinant human OTX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-174 aa of BC007621 Sequence: VSSSASSSSSASSSSANPAAAAAAGLGGNPVAAASSLSTPAAS 相同性解析による交差性が予測される生物種 |
| Full Name | orthodenticle homeobox 1 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 37-43 kDa |
| GenBank accession number | BC007621 |
| Gene Symbol | OTX1 |
| Gene ID (NCBI) | 5013 |
| RRID | AB_2880568 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32242 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OTX1, also named as Orthodenticle homolog 1, is a 354 amino acid protein, which belongs to the paired homeobox family. OTX1 is expressed in brain and detected in the anterior part of the neural fetal retina. OTX1 probably plays a role in the development of the brain and the sense organs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTX1 antibody 26595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
