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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using OTX1 Polyclonal antibody (26595-1-AP)

    WB analysis of HEK-293 using 26595-1-AP

    WB result of OTX1 antibody (26595-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTX1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of COLO 320 cells using OTX1 Polyclonal antibody (26595-1-AP)

    WB analysis of COLO 320 using 26595-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26595-1-AP (OTX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using OTX1 Polyclonal antibody (26595-1-AP)

    WB analysis of COLO 320 using 26595-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26595-1-AP (OTX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

OTX1 Polyclonal antibody

OTX1 Polyclonal Antibody for WB, ELISA
Cat No. 26595-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Homeobox protein OTX1, orthodenticle homeobox 1, Orthodenticle homolog 1, OTX1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26595-1-AP targets OTX1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTX1 fusion protein Ag24252 相同性解析による交差性が予測される生物種
Full Name orthodenticle homeobox 1
Calculated molecular weight 37 kDa
Observed molecular weight 37-43 kDa
GenBank accession numberBC007621
Gene Symbol OTX1
Gene ID (NCBI) 5013
RRIDAB_2880568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32242
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OTX1, also named as Orthodenticle homolog 1, is a 354 amino acid protein, which belongs to the paired homeobox family. OTX1 is expressed in brain and detected in the anterior part of the neural fetal retina. OTX1 probably plays a role in the development of the brain and the sense organs.

Protocols

Product Specific Protocols
WB protocol for OTX1 antibody 26595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols