Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86490-1-RR targets OTUD5 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15190 Product name: Recombinant human OTUD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-561 aa of BC028225 Sequence: WFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVPCRC 相同性解析による交差性が予測される生物種 |
| Full Name | OTU domain containing 5 |
| Calculated molecular weight | 571 aa, 61 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC028225 |
| Gene Symbol | OTUD5 |
| Gene ID (NCBI) | 55593 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96G74 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD5 antibody 86490-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |