Validation Data Gallery
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Tested Applications
| Positive WB detected in | Daudi cells, Raji cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
25070-1-AP targets OTUD4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OTUD4 fusion protein Ag18401 相同性解析による交差性が予測される生物種 |
| Full Name | OTU domain containing 4 |
| Calculated molecular weight | 1113 aa, 124 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | BC118653 |
| Gene Symbol | OTUD4 |
| Gene ID (NCBI) | 54726 |
| RRID | AB_2879882 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01804 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD4 antibody 25070-1-AP | Download protocol |
| IHC protocol for OTUD4 antibody 25070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway | ||
Cell Death Differ N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors | ||
Front Microbiol MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.
| ||
Dev Cell Glutamine binds HSC70 to transduce signals inhibiting IFN-β-mediated immunogenic cell death
| ||
Exp Cell Res Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1
| ||
PLoS Pathog Non-canonical regulation of the reactivation of an oncogenic herpesvirus by the OTUD4-USP7 deubiquitinases |