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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTUD1 Polyclonal antibody (29921-1-AP)

    WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    WB analysis of mouse colon using 29921-1-AP (same clone as 29921-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using OTUD1 Polyclonal antibody (29921-1-AP)

    WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using OTUD1 Polyclonal antibody (29921-1-AP)

    IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

OTUD1 Polyclonal antibody, PBS Only

OTUD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29921-1-PBS targets OTUD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31996

Product name: Recombinant human OTUD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 272-431 aa of

Sequence: SSAAEPVIVSRSDPRDEKLALYLAEVEKQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGH

相同性解析による交差性が予測される生物種
Full Name OTU domain containing 1
Calculated molecular weight51 kDa
Observed molecular weight50-55 kDa
Gene Symbol OTUD1
Gene ID (NCBI) 220213
RRIDAB_3086190
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VV17
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

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