Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33794-1-AP targets OTOG in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg5591 Product name: Recombinant human OTOG protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1634-1796 aa of NM_001277269.2 Sequence: VRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAA 相同性解析による交差性が予測される生物種 |
| Full Name | otogelin |
| Calculated molecular weight | 315 kDa |
| Observed molecular weight | 314 kDa |
| GenBank accession number | NM_001277269.2 |
| Gene Symbol | OTOG |
| Gene ID (NCBI) | 340990 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6ZRI0-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OTOG (Otogelin) is a glycoprotein that is highly expressed in the inner ear and is essential for maintaining hearing and balance functions. It belongs to the mucin-like glycoprotein family and is a large extracellular matrix protein with numerous glycosylation sites. This allows it to form gel-like structures that provide structural support during mechanical transduction in the inner ear. Mutations or loss of function in the OTOG gene can lead to severe hereditary deafness and vestibular dysfunction. There are two isoforms of OTOG, with molecular weights of 160 kDa and 314 kDa respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTOG antibody 33794-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |