Validation Data Gallery
Tested Applications
| Positive WB detected in | 37°C incubated mouse brain tissue, mouse brain membrane protein |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
14621-1-AP targets OSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6254 Product name: Recombinant human OSTM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 38-282 aa of BC068581 Sequence: SPHRVFHDLLSEQQLLEVEDLSLSLLQGGGLGPLSLPPDLPDLDPECRELLLDFANSSAELTGCLVRSARPVRLCQTCYPLFQQVVSKMDNISRAAGNTSESQSCARSLLMADRMQIVVILSEFFNTTWQEANCANCLTNNSEELSNSTVYFLNLFNHTLTCFEHNLQGNAHSLLQTKNYSEVCKNCREAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVPCSDT 相同性解析による交差性が予測される生物種 |
| Full Name | osteopetrosis associated transmembrane protein 1 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | ~ 60 kDa |
| GenBank accession number | BC068581 |
| Gene Symbol | OSTM1 |
| Gene ID (NCBI) | 28962 |
| RRID | AB_2298955 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OSTM1 is a type I transmembrane protein. Ostm1 is essential for osteoclast maturation and is responsible for the most severe forms of osteoporosis when mutated in mice and humans. The OSTM1 protein has 10 N-glycosylation sites and has an apparent mass of ~60 kDa after being highly N-glycosylated (PMID: 26598607; PMID: 32764302). The OSTM1 protein can also be detected at ~30-45 kDa and ~70-80 kDa in some literatures (PMID: 16525474; PMID: 24159188;PMID: 20830208).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OSTM1 antibody 14621-1-AP | Download protocol |
| IHC protocol for OSTM1 antibody 14621-1-AP | Download protocol |
| WB protocol for OSTM1 antibody 14621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Virol Osteopetrosis-Associated Transmembrane Protein 1 Recruits RNA Exosome To Restrict Hepatitis B Virus Replication. | ||
Biology (Basel) Leucine Repeat Rich Kinase 1 Controls Osteoclast Activity by Managing Lysosomal Trafficking and Secretion |
