Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
31330-1-AP targets OSTA in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34517 Product name: Recombinant human SLC51A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-48 aa of NM_152672 Sequence: MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGP 相同性解析による交差性が予測される生物種 |
| Full Name | organic solute transporter alpha |
| Calculated molecular weight | 340 aa, 38 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | NM_152672 |
| Gene Symbol | SLC51A |
| Gene ID (NCBI) | 200931 |
| RRID | AB_3669946 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UW1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC51A also known as OSTA, belongs to the OST-alpha family. SLC51A is an essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood (PubMed:16317684). SLC51A is located in basolateral plasma membrane and transported from the endoplasmic reticulum to the plasma membrane upon interacting with SLC51B. SLC51A is expressed with a high expression in kidney and intestine (PMID: 15563450). Mutations in SLC51A are associated with Cholestasis progressive familial intrahepatic 6(PMID: 31863603).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OSTA antibody 31330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |