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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis using 15033-1-AP (same clone as 15033-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis of Raji using 15033-1-AP (same clone as 15033-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis of human heart using 15033-1-AP (same clone as 15033-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human kidney using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human pancreas cancer using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human pancreas cancer using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human kidney using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human heart using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human heart using 15033-1-AP (same clone as 15033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15033-1-PBS in a different storage buffer formulation.

OSGEP Polyclonal antibody, PBS Only

OSGEP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15033-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15033-1-PBS targets OSGEP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4308

Product name: Recombinant human OSGEP protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-335 aa of BC032310

Sequence: MPAVLGFEGSANKIGVGVVRDGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTESGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGATSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMDVSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVGGVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAGWEMFRAGHRTPLSDSGVTQRYRTDEVEVTWRD

相同性解析による交差性が予測される生物種
Full Name O-sialoglycoprotein endopeptidase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC032310
Gene Symbol OSGEP
Gene ID (NCBI) 55644
RRIDAB_2283171
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPF4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).

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