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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSBPL5 Polyclonal antibody (27870-1-AP)

    WB analysis using 27870-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27870-1-AP (OSBPL5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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OSBPL5 Polyclonal antibody

OSBPL5 Polyclonal Antibody for WB, ELISA
Cat No. 27870-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

OSBP related protein 5, ORP-5, ORP5, ORP 5, OBPH1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27870-1-AP targets OSBPL5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSBPL5 fusion protein Ag27304 相同性解析による交差性が予測される生物種
Full Name oxysterol binding protein-like 5
Calculated molecular weight 811 aa, 91 kDa
Observed molecular weight100 kDa
GenBank accession numberBC032646
Gene Symbol OSBPL5
Gene ID (NCBI) 114879
RRIDAB_3669627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0X9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OSBPL5, also known as oxysterol binding protein like 5, is a protein-coding gene in humans. It is a member of the oxysterol-binding protein (OSBP) family, which consists of intracellular lipid receptors that play a crucial role in maintaining cholesterol balance in the body. It is expressed ubiquitously in various tissues, including the spleen and endometrium. OSBPL5 is classified as a plasma protein and transporter, with a predicted subcellular location in the membrane and intracellular compartments. The protein has been implicated in several biological processes and is involved in the transport of lipids and other molecules between organelles. In terms of disease association, OSBPL5 has been linked to conditions such as Lenz-Majewski hyperostotic dwarfism. Additionally, the OSBPL family of proteins, including OSBPL5, has been suggested to play a role in cancer development, although specific roles in different types of cancer are still being investigated (PMID: 36918840). The molecular weight of OSBPL5 is 98 kDa.

Protocols

Product Specific Protocols
WB protocol for OSBPL5 antibody 27870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols