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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSBPL3 Polyclonal antibody (12417-1-AP)

    WB analysis using 12417-1-AP (same clone as 12417-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12417-1-AP (OSBPL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using OSBPL3 Polyclonal antibody (12417-1-AP)

    WB analysis of HCT 116 using 12417-1-AP (same clone as 12417-1-PBS)

    WB result of OSBPL3 antibody (12417-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OSBPL3 transfected HCT 116 cells. This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using OSBPL3 Polyclonal antibody (12417-1-AP)

    WB analysis of K-562 using 12417-1-AP (same clone as 12417-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12417-1-AP (OSBPL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of human colon cancer using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of human colon cancer using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of human colon cancer using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of human colon cancer using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of mouse kidney using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of human ovary cancer using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of mouse bladder using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat bladder tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of rat bladder using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using OSBPL3 Polyclonal antibody (12417-1-AP)

    IHC staining of rat kidney using 12417-1-AP (same clone as 12417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12417-1-AP (OSBPL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12417-1-PBS in a different storage buffer formulation.

OSBPL3 Polyclonal antibody, PBS Only

OSBPL3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12417-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0704, ORP 3, ORP3, ORP-3, OSBP related protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12417-1-PBS targets OSBPL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3044

Product name: Recombinant human OSBPL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC017731

Sequence: MMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQDSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWPLKGWHKRFFYLDKGILKYAKSQTDIEREKLHGCIDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEEVFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSGSTITDSSSGVFDSISSRKRSSISKQNLFQTGSNVSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAYLVEMSQLLQSMDVLHRTYSAPAINAIQGGSFESPKKEKRSHRRWRSRAIGKDAKGTLQVPKPFSGPVRLHSSNPNLSTLDFGEEKNYSDGSETSSEFSKMQEDLCHIAHKVYFTLRSA

相同性解析による交差性が予測される生物種
Full Name oxysterol binding protein-like 3
Calculated molecular weight 887 aa, 101 kDa
Observed molecular weight 95-110 kDa
GenBank accession numberBC017731
Gene Symbol OSBPL3
Gene ID (NCBI) 26031
RRIDAB_2156103
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4L5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OSBPL3 (ORP3) is an oxysterol-binding protein-related lipid transfer protein located at ER-plasma membrane contact sites. Its canonical isoform contains 887 amino acids with a theoretical molecular weight of 101.2 kDa. Western blot routinely detects a doublet around 103 kDa and 110 kDa, mainly caused by differential phosphorylation rather than splicing variants. It interacts with R-Ras and VAPA to modulate PI4P transport, actin cytoskeleton dynamics and cell adhesion. Aberrant OSBPL3 expression drives proliferation and metastasis in multiple cancers, acting as a prognostic biomarker and potential therapeutic target. (PMID: 32041906, 34584127)

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