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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ORM1/2 Polyclonal antibody (11199-1-AP)

    WB analysis using 11199-1-AP (same clone as 11199-1-PBS)

    human plasma lysates were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ORM1/2 Polyclonal antibody (11199-1-AP)

    WB analysis using 11199-1-AP (same clone as 11199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using ORM1/2 Polyclonal antibody (11199-1-AP)

    IP experiment of L02 using 11199-1-AP (same clone as 11199-1-PBS)

    IP result of anti-ORM1/2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1/2 Polyclonal antibody (11199-1-AP)

    IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1/2 Polyclonal antibody (11199-1-AP)

    IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ORM1/2 Polyclonal antibody (11199-1-AP)

    IF Staining of HEK-293 using 11199-1-AP (same clone as 11199-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ORM1/2 antibody (11199-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

ORM1/2 Polyclonal antibody, PBS Only

ORM1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ORM2, AGP 2, AGP B, AGP B', AGP2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11199-1-PBS targets ORM1/2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1667

Product name: Recombinant human ORM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-201 aa of BC015964

Sequence: MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHVAHLLFLRDTKTLMFGSYLDDEKNWGLSFYADKPETTKEQLGEFYEALDCLCIPRSDVMYTDWKKDKCEPLEKQHEKERKQEEGES

相同性解析による交差性が予測される生物種
Full Name orosomucoid 2
Calculated molecular weight 24 kDa
Observed molecular weight 24-28 kDa, 41-47 kDa
GenBank accession numberBC015964
Gene Symbol ORM2
Gene ID (NCBI) 5005
RRIDAB_2158180
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19652
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.

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