Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32429-1-AP targets ORC3L in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ORC3L fusion protein Ag37481 相同性解析による交差性が予測される生物種 |
| Full Name | origin recognition complex, subunit 3-like (yeast) |
| Calculated molecular weight | 66 kDa, 82 kDa |
| Observed molecular weight | 66 kDa, 82 kDa |
| GenBank accession number | NM_012381.3 |
| Gene Symbol | ORC3L |
| Gene ID (NCBI) | 23595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9UBD5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ORC3L, also known as Origin Recognition Complex Subunit 3-like, is a human gene that encodes a protein which is a subunit of the Origin Recognition Complex (ORC). The ORC is a highly conserved six-subunit protein complex that is essential for the initiation of DNA replication in eukaryotic cells. Studies in yeast have shown that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ORC3L antibody 32429-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |