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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ORC2L Polyclonal antibody

ORC2L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12739-1-AP

Synonyms

ORC2, ORC2L

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12739-1-AP

    WB analysis of HepG2 using 12739-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12739-1-AP (ORC2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12739-1-AP

    IP experiment of HepG2 using 12739-1-AP

    IP result of anti-ORC2L (IP:12739-1-AP, 4ug; Detection:12739-1-AP 1:300) with HepG2 cells lysate 1200ug.

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

12739-1-AP targets ORC2L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORC2L fusion protein Ag3635 相同性解析による交差性が予測される生物種
Full Name origin recognition complex, subunit 2-like (yeast)
Calculated molecular weight 577 aa, 66 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC014834
Gene symbol ORC2L
Gene ID (NCBI) 4999
RRIDAB_10640799
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ORC2L antibody 12739-1-APDownload protocol
IP protocol for ORC2L antibody 12739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols