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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ORAI2 Polyclonal antibody (20592-1-AP)

    WB analysis of MCF-7 using 20592-1-AP (same clone as 20592-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ORAI2 Polyclonal antibody (20592-1-AP)

    WB analysis of mouse heart using 20592-1-AP (same clone as 20592-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ORAI2 Polyclonal antibody (20592-1-AP)

    IHC staining of human kidney using 20592-1-AP (same clone as 20592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ORAI2 Polyclonal antibody (20592-1-AP)

    IHC staining of rat brain using 20592-1-AP (same clone as 20592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20592-1-AP (ORAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ORAI2 Polyclonal antibody (20592-1-AP)

    IF Staining of HEK-293 using 20592-1-AP (same clone as 20592-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ORAI2 antibody (20592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

ORAI2 Polyclonal antibody, PBS Only

ORAI2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20592-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C7orf19, CAP-binding protein complex-interacting protein 2, CBCIP2, PP1729, Protein orai 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20592-1-PBS targets ORAI2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14540

Product name: Recombinant human ORAI2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-254 aa of BC069270

Sequence: VDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL

相同性解析による交差性が予測される生物種
Full Name ORAI calcium release-activated calcium modulator 2
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC069270
Gene Symbol ORAI2
Gene ID (NCBI) 80228
RRIDAB_2878705
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96SN7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

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