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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN5 Polyclonal antibody (25251-1-AP)

    IF Staining of mouse eye using 25251-1-AP (same clone as 25251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN5 Polyclonal antibody (25251-1-AP)

    IF Staining of mouse eye using 25251-1-AP (same clone as 25251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25251-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25251-1-PBS targets OPN5 in IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPN5 fusion protein Ag19384 相同性解析による交差性が予測される生物種
Full Name opsin 5
Calculated molecular weight 354 aa, 40 kDa
GenBank accession numberBC126198
Gene Symbol OPN5
Gene ID (NCBI) 221391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6U736
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuropsin (OPN5) is an opsin family member known as a photopigment responsive to wavelengths in the near-UV (λmax = 380 nm) (PMID: 31607531). In mammals, OPN5 is required to photoentrainment the local circadian oscillator in the murine retina and cornea (PMID: 21135214).