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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN5 Polyclonal antibody (25251-1-AP)

    IF Staining of mouse eye using 25251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using OPN5 Polyclonal antibody (25251-1-AP)

    IF Staining of mouse eye using 25251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using OPN5 antibody (25251-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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OPN5 Polyclonal antibody

OPN5 Polyclonal Antibody for IF-P, ELISA
Cat No. 25251-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, ELISA

Synonyms

TMEM13, PGR12, opsin 5, NEUROPSIN, GPR136

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25251-1-AP targets OPN5 in IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPN5 fusion protein Ag19384 相同性解析による交差性が予測される生物種
Full Name opsin 5
Calculated molecular weight 354 aa, 40 kDa
GenBank accession numberBC126198
Gene Symbol OPN5
Gene ID (NCBI) 221391
RRIDAB_3669465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6U736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuropsin (OPN5) is an opsin family member known as a photopigment responsive to wavelengths in the near-UV (λmax = 380 nm) (PMID: 31607531). In mammals, OPN5 is required to photoentrainment the local circadian oscillator in the murine retina and cornea (PMID: 21135214).

Protocols

Product Specific Protocols
IF protocol for OPN5 antibody 25251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols