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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OPN4 Polyclonal antibody

OPN4 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 24478-1-AP

Synonyms

Melanopsin, MOP, OPN4, opsin 4

Validation Data Gallery

Applications:
  • IF Staining of mouse eye using 24478-1-AP

    IF Staining of mouse eye using 24478-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN4 antibody (24478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

24478-1-AP targets OPN4 in IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPN4 fusion protein Ag21643 相同性解析による交差性が予測される生物種
Full Name opsin 4
Calculated molecular weight 478 aa, 53 kDa
GenBank accession numberBC113558
Gene symbol OPN4
Gene ID (NCBI) 94233
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OPN4, an opsin that was first isolated from the skin of Xenopus laevis in 1998, belongs not to the vertebrate-type opsin family but rather to the invertebrate-type opsins, which are part of the seven-transmembrane G protein-coupled receptor (GPCR) family (PMID:31989708). In humans, OPN4 is expressed in the retinal ganglion cells in addition to part of the brain (PMID:10632589). Moreover, Opn4 functions as a photoreceptor by detecting brightness levels and discriminating visual signals (PMID:22633808). Opn4 also facilitates visual circuits to acquire optimal settings and light adaptation by measuring irradiance levels (PMID:25517373).

Protocols

Product Specific Protocols
IF protocol for OPN4 antibody 24478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols