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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

OPLAH Polyclonal antibody

OPLAH Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21127-1-AP

Synonyms

5 Opase, 5 oxo L prolinase, 5 oxoprolinase, OPLA, OPLAH, Pyroglutamase

Validation Data Gallery

Applications:
  • WB analysis using 21127-1-AP

    WB analysis using 21127-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 21127-1-AP

    WB analysis of L02 using 21127-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse bladder using 21127-1-AP

    WB analysis of mouse bladder using 21127-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 21127-1-AP

    WB analysis of mouse brain using 21127-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, L02 cells, mouse bladder tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21127-1-AP is OPLAH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPLAH fusion protein Ag15523 相同性解析による交差性が予測される生物種
Full Name 5-oxoprolinase (ATP-hydrolysing)
Calculated molecular weight 1288 aa, 137 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC142672
Gene symbol OPLAH
Gene ID (NCBI) 26873
RRIDAB_10733639
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OPLAH antibody 21127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

Authors - Kenneth N Maclean
View Article