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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPLAH Polyclonal antibody (21127-1-AP)

    WB analysis using 21127-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse bladder tissue using OPLAH Polyclonal antibody (21127-1-AP)

    WB analysis of mouse bladder using 21127-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using OPLAH Polyclonal antibody (21127-1-AP)

    WB analysis of L02 using 21127-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using OPLAH Polyclonal antibody (21127-1-AP)

    WB analysis of mouse brain using 21127-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21127-1-AP (OPLAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

OPLAH Polyclonal antibody

OPLAH Polyclonal Antibody for WB, ELISA
Cat No. 21127-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

5 Opase, 5 oxo L prolinase, 5 oxoprolinase, OPLA, OPLAH, Pyroglutamase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue, L02 cells, mouse bladder tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21127-1-AP targets OPLAH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15523

Product name: Recombinant human OPLAH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 939-1288 aa of BC142672

Sequence: ELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGLPLEVSSEDHMDDGSPIRLRVQISLSQGSAVFDFSGTGPEVFGNLNAPRAVTLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPRGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPSWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRRGSGGRGRFRGGDGVTRELLFREEALLSVLTERRAFRPYGLHGGEPGARGLNLLIRKNGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPAPPPGSPPQALAFPEHGSVYEYRRAQEAV

相同性解析による交差性が予測される生物種
Full Name 5-oxoprolinase (ATP-hydrolysing)
Calculated molecular weight 1288 aa, 137 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC142672
Gene Symbol OPLAH
Gene ID (NCBI) 26873
RRIDAB_10733639
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for OPLAH antibody 21127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

Authors - Kenneth N Maclean
View Article
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