Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87815-1-RR targets OPCML in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6020 Product name: Recombinant human OPCM protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 28-322 aa of NM_001012393.5 Sequence: DATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALA 相同性解析による交差性が予測される生物種 |
| Full Name | opioid binding protein/cell adhesion molecule-like |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 40 kDa, 60 kDa |
| GenBank accession number | NM_001012393.5 |
| Gene Symbol | OPCML |
| Gene ID (NCBI) | 4978 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14982-2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OPCML, also called opioid-binding cell adhesion molecule (OBCAM), belongs to the IgLON limbic system-associated membrane protein (LSAMP, OPCML/OBCAM, neurotrimin) family of IgG domain-containing glycosylphosphatidylinositol-anchored cell adhesion molecules. Originally isolated from the brain, OPCML is also expressed in the stomach, ovary, heart, placenta, testis, kidney, liver, pancreas, and colon. OPCML has been identified as a tumour suppressor, which inhibited tumor growth in some cancers, including ovarian cancer, lung cancer, and prostate cancer (PMID: 19176311; 28407749).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OPCML antibody 87815-1-RR | Download protocol |
| WB protocol for OPCML antibody 87815-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |