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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OPCML Polyclonal antibody (17962-1-AP)

    WB analysis using 17962-1-AP (same clone as 17962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17962-1-AP (OPCML antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using OPCML Polyclonal antibody (17962-1-AP)

    IHC staining of rat brain using 17962-1-AP (same clone as 17962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17962-1-AP (OPCML antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using OPCML Polyclonal antibody (17962-1-AP)

    IHC staining of rat brain using 17962-1-AP (same clone as 17962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17962-1-AP (OPCML antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using OPCML Polyclonal antibody (17962-1-AP)

    IF Staining of mouse brain using 17962-1-AP (same clone as 17962-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OPCML antibody (17962-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17962-1-PBS in a different storage buffer formulation.

OPCML Polyclonal antibody, PBS Only

OPCML Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 17962-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

IgLON family member 1, IGLON1, OBCAM, Opioid-binding cell adhesion molecule, Opioid-binding protein/cell adhesion molecule

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17962-1-PBS targets OPCML in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12560

Product name: Recombinant human OPCML protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-345 aa of BC074773

Sequence: TGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALACLWLSGTLLAHFFIKF

相同性解析による交差性が予測される生物種
Full Name opioid binding protein/cell adhesion molecule-like
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight45 kDa
GenBank accession numberBC074773
Gene Symbol OPCML
Gene ID (NCBI) 4978
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14982
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OPCML, also called opioid-binding cell adhesion molecule (OBCAM), belongs to the IgLON limbic system-associated membrane protein (LSAMP, OPCML/OBCAM, neurotrimin) family of IgG domain containing glycosylphosphatidylinositol-anchored cell adhesion molecules. Originally isolated from brain, OPCMLis also expressed in stomach, ovary, heart, placenta, testis, kidney, liver, pancreas and colon. OPCML has been identified as a tumour suppressor, which inhibited tumor growth in some cancers including ovarian cancer, lung cancer and prostate cancer (PMID: 19176311; 28407749).

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