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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    WB analysis of mouse liver using 25137-1-AP (same clone as 25137-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ONECUT1 Polyclonal antibody (25137-1-AP)

    IHC staining of human liver using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

ONECUT1 Polyclonal antibody, PBS Only

ONECUT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25137-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25137-1-PBS targets ONECUT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18250

Product name: Recombinant human ONECUT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 167-292 aa of BC140830

Sequence: PYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTDKMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQVSNGSNSGQMEEI

相同性解析による交差性が予測される生物種
Full Name one cut homeobox 1
Calculated molecular weight 465 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC140830
Gene Symbol ONECUT1
Gene ID (NCBI) 3175
RRIDAB_2879918
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

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