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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of PC-3 using 12596-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of mouse liver using 12596-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of A2780 using 12596-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of human liver using 12596-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    WB analysis of NIH/3T3 using 12596-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using OLFM3 Polyclonal antibody (12596-1-AP)

    IHC staining of human liver cancer using 12596-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12596-1-AP (OLFM3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OLFM3 Polyclonal antibody (12596-1-AP)

    IF Staining of HepG2 using 12596-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OLFM3 antibody (12596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

OLFM3 Polyclonal antibody

OLFM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12596-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Olfactomedin 3, NOE3, Noelin 3, NOELIN3, Noelin-3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inPC-3 cells, A2780 cells, human liver tissue, mouse liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12596-1-AP targets OLFM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3207

Product name: Recombinant human OLFM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-458 aa of BC022531

Sequence: NLSAVLTGIQEEIGAYDYEELHQRVLSLETRLRDCMKKLTCGKLMKITGPVTVKTSGTRFGAWMTDPLASEKNNRVWYMDSYTNNKIVREYKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMGRVLAQRSLEYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLEVMKSWSTGYPKRSAGESFMICGTLYVTNSHLTGAKVYYSYSTKTSTYEYTDIPFHNQYFHISMLDYNARDRALCAWNNGHQVLFNVTLFHIIKTEDDT

相同性解析による交差性が予測される生物種
Full Name olfactomedin 3
Calculated molecular weight 458 aa, 53 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC022531
Gene Symbol OLFM3
Gene ID (NCBI) 118427
RRIDAB_2157228
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OLFM3, also known as Noelin-3, belongs to a larger family of proteins containing the olfactomedin domain. OLFM3, which is most closely related to OLFM2, is expressed in the retinal ganglion and inner nuclear layers of the adult rat retina (PMID: 12019210).

Protocols

Product Specific Protocols
IF protocol for OLFM3 antibody 12596-1-APDownload protocol
IHC protocol for OLFM3 antibody 12596-1-APDownload protocol
WB protocol for OLFM3 antibody 12596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Front Cell Dev Biol

Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models.

Authors - Shirong Tang
View Article
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