Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 19842-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19842-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 19842-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19842-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19842-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 19842-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19842-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 19842-1-AP
IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of mouse lung using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 19842-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 19842-1-AP (hIST1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 19842-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using hIST1 antibody (19842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19842-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Synonyms

IST1, KIAA0174, Charged multivesicular body protein 8, CHMP8, IST1 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
Positive IHC detected in	mouse stomach tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IF	See 10 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

19842-1-AP targets hIST1 in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13874 Product name: Recombinant human hIST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC000116 Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT 相同性解析による交差性が予測される生物種
Full Name	KIAA0174
Calculated molecular weight	364 aa, 40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC000116
Gene Symbol	hIST1
Gene ID (NCBI)	9798
RRID	AB_2878612
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P53990
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for hIST1 antibody 19842-1-AP	Download protocol
IHC protocol for hIST1 antibody 19842-1-AP	Download protocol
IP protocol for hIST1 antibody 19842-1-AP	Download protocol
WB protocol for hIST1 antibody 19842-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
	IF	Science Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Authors - Michael L Skowyra View Article
human	IF	Cell Res Repair of damaged lysosomes by TECPR1-mediated membrane tubulation during energy crisis. Authors - Hanmo Chen View Article
human	WB,IF,CoIP	Autophagy ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms Authors - Zhijie Xu View Article
		Nat Commun VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair. Authors - Oluwatobi Andrew Adeosun View Article
human	IF	Nat Commun Impaired α -Synuclein aggregate clearance in neuronal cells drive their spread to microglia through tunneling nanotubes. Authors - Ranabir Chakraborty View Article

Validation Data Gallery

WB analysis using 19842-1-AP

WB analysis of HeLa using 19842-1-AP

WB analysis of mouse liver using 19842-1-AP

WB analysis of mouse brain using 19842-1-AP

WB analysis of mouse brain using 19842-1-AP

WB analysis of PC-3 using 19842-1-AP

WB analysis of HeLa using 19842-1-AP

IP experiment of mouse liver using 19842-1-AP

IHC staining of mouse lung using 19842-1-AP

IHC staining of mouse stomach using 19842-1-AP

IHC staining of mouse stomach using 19842-1-AP

IF Staining of HepG2 using 19842-1-AP

hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Repair of damaged lysosomes by TECPR1-mediated membrane tubulation during energy crisis.

ATP6V1D drives hepatocellular carcinoma stemness and progression via both lysosome acidification-dependent and -independent mechanisms

VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair.

Impaired α -Synuclein aggregate clearance in neuronal cells drive their spread to microglia through tunneling nanotubes.

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