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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OLAH Polyclonal antibody (17676-1-AP)

    WB analysis using 17676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17676-1-AP (OLAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using OLAH Polyclonal antibody (17676-1-AP)

    WB analysis using 17676-1-AP

    MCF-10A cells were subjected to SDS PAGE followed by western blot with 17676-1-AP (OLAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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OLAH Polyclonal antibody

OLAH Polyclonal Antibody for WB, ELISA
Cat No. 17676-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Augmented in rheumatoid arthritis 1, AURA1, EC:3.1.2.14, Oleoyl-ACP hydrolase, S-acyl fatty acid synthase thioesterase, medium chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, MCF-10A cells, LNCap cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17676-1-AP targets OLAH in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLAH fusion protein Ag11694 相同性解析による交差性が予測される生物種
Full Name oleoyl-ACP hydrolase
Calculated molecular weight 265 aa, 30 kDa
Observed molecular weight36 kDa
GenBank accession numberBC050372
Gene Symbol OLAH
Gene ID (NCBI) 55301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OLAH protein, also known as oleoyl-ACP hydrolase, is an enzyme involved in fatty acid production. It has been found to play a role in various biological processes and diseases. For instance, placental OLAH levels are altered in fetal growth restriction and preeclampsia, suggesting its potential involvement in placental dysfunction. Additionally, OLAH has been linked to severe respiratory diseases, where it mediates life-threatening inflammation associated with viral infections. Its expression is also associated with fatal outcomes in certain viral infections. These findings highlight the importance of OLAH in both reproductive health and infectious diseases (PMID: 36139751, PMID: 39137778, PMID: 39178832). OLAH has two isoforms with molecular masses of 30 kDa and 36 kDa.

Protocols

Product Specific Protocols
WB protocol for OLAH antibody 17676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols