Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 16371-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16371-1-AP
WB result of GTPBP9 antibody (16371-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GTPBP9 transfected HepG2 cells.
WB analysis of HepG2 using 16371-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 16371-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 16371-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

GTPBP9 Polyclonal antibody

GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16371-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

OLA1, Obg-like ATPase 1, Obg like ATPase 1, GTP-binding protein 9, GTP binding protein 9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, MCF-7 cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IF	See 1 publications below

Product Information

16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9283 Product name: Recombinant human OLA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-250 aa of BC013925 Sequence: MPPKKGGDGIKPPPIIGRFGTSLKIGIVGLPNVGKSTFFNVLTNSQASAENFPFCTIDPNESRVPVPDERFDFLCQYHKPASKIPAFLNVVDIAGLVKGAHNGQGLGNAFLSHISACDGIFHLTRAFEDDDITHVEGSVDPIRDIEIIHEELQLKDEEMIGPIIDKLEKVAVRGGDKKLKPEYDIMCKVKSWVIDQKKPVRFYHDWNDKEIEVLNKHLFLTSKPMVYLVNLSEKDYIRKKNKWLLESTDN 相同性解析による交差性が予測される生物種
Full Name	Obg-like ATPase 1
Calculated molecular weight	396aa,45 kDa; 278aa,31 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC013925
Gene Symbol	OLA1
Gene ID (NCBI)	29789
RRID	AB_2157087
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NTK5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for GTPBP9 antibody 16371-1-AP	Download protocol
IHC protocol for GTPBP9 antibody 16371-1-AP	Download protocol
WB protocol for GTPBP9 antibody 16371-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	bioRxiv Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart Authors - Praveen K Dubey View Article
mouse	WB	iScience Aged gut microbiota promotes arrhythmia susceptibility via oxidative stress Authors - Zhi-Ping Fu View Article
human	WB	Clin Exp Pharmacol Physiol OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling. Authors - Gayathri Narasimhan KD Validated View Article
human,mouse	WB	medRxiv Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart Authors - Praveen K Dubey View Article
human	WB	PLoS One Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart Authors - Praveen K Dubey View Article
human	WB	J Cell Sci HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis Authors - Lianfei Zhang KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 16371-1-AP

WB analysis of HepG2 using 16371-1-AP

WB analysis of HepG2 using 16371-1-AP

IHC staining of human liver cancer using 16371-1-AP

IHC staining of human liver cancer using 16371-1-AP

IHC staining of human colon cancer using 16371-1-AP

IHC staining of human colon cancer using 16371-1-AP

IF Staining of MCF-7 using 16371-1-AP

GTPBP9 Polyclonal antibody

GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart

Aged gut microbiota promotes arrhythmia susceptibility via oxidative stress

OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.

Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart

Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart

HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis