Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32301-1-AP targets OIT3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OIT3 fusion protein Ag36932 相同性解析による交差性が予測される生物種 |
| Full Name | oncoprotein induced transcript 3 |
| Calculated molecular weight | 60 kDa，545aa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | NM_152635.2 |
| Gene Symbol | OIT3 |
| Gene ID (NCBI) | 170392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WWZ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Oncoprotein-induced transcript 3 protein (OIT3) is a liver-specific protein. It is involved in various biological processes, including lipid metabolism and ferroptosis regulation. OIT3 has been shown to mediate macrophage polarization and facilitate hepatocellular carcinoma (HCC) progression by regulating the arachidonic acid metabolism and reactive oxygen species (ROS) accumulation (PMID: 35353239). Additionally, OIT3 is implicated in the regulation of lipid metabolism, particularly in the context of triacylglycerol and very low-density lipoproteins (VLDLs) in the liver (PMID: 36132142).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OIT3 antibody 32301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |