• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of human heart using 66285-1-Ig (same clone as 66285-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of ROS1728 using 66285-1-Ig (same clone as 66285-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OGDH Monoclonal antibody (66285-1-Ig)

    IF Staining of HepG2 using 66285-1-Ig (same clone as 66285-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66285-1-PBS in a different storage buffer formulation.

OGDH Monoclonal antibody, PBS Only

OGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66285-1-PBS
Clone No.1H7A2

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66285-1-PBS targets OGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7484

Product name: Recombinant human OGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 674-1023 aa of BC004964

Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS

相同性解析による交差性が予測される生物種
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_2881668
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

||
New chat Able
正在加载，请稍候...