Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15212-1-AP
WB result of OGDH antibody (15212-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OGDH transfected HeLa cells.
WB analysis using 15212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 15212-1-AP
ROS1728 cells were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15212-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 15212-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15212-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 15212-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15212-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15212-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells stained for OGDH rabbit pAb (15212-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15212-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG (H+L) for 66031-1-Ig. .
IF Staining of A549 using 15212-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using OGDH antibody (15212-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

OGDH Polyclonal antibody

OGDH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15212-1-AP

Publications(76)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, Alpha-ketoglutarate dehydrogenase, Alpha-KGDH-E1, E1o

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (76)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, A549 cells, HeLa cells, mouse liver tissue, mouse pancreas tissue, ROS1728 cells, rat heart tissue, mouse lung tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 70 publications below
IHC	See 6 publications below
IF	See 5 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

15212-1-AP targets OGDH in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit, chicken, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7368 Product name: Recombinant human OGDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 674-1023 aa of BC004964 Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS 相同性解析による交差性が予測される生物種
Full Name	oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated molecular weight	116 kDa
Observed molecular weight	116 kDa
GenBank accession number	BC004964
Gene Symbol	OGDH
Gene ID (NCBI)	4967
RRID	AB_2156759
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q02218
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Protocols

Product Specific Protocols
IF protocol for OGDH antibody 15212-1-AP	Download protocol
IHC protocol for OGDH antibody 15212-1-AP	Download protocol
WB protocol for OGDH antibody 15212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IHC	Nature α-Ketoglutarate links p53 to cell fate during tumour suppression. Authors - John P Morris KD Validated View Article
	WB	Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. Authors - Qiuying Chen View Article
mouse,human	WB	Cell Metab An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism. Authors - Nunu Huang View Article
human	WB	Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Authors - Jia-Yuan Zhang View Article
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB	J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. Authors - Weiqi Dai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15212-1-AP

WB analysis of HeLa using 15212-1-AP

WB analysis using 15212-1-AP

WB analysis of ROS1728 using 15212-1-AP

WB analysis of rat lung using 15212-1-AP

WB analysis of mouse pancreas using 15212-1-AP

WB analysis of mouse liver using 15212-1-AP

IHC staining of human lung cancer using 15212-1-AP

IHC staining of human lung cancer using 15212-1-AP

IF Staining of HeLa using 15212-1-AP

IF Staining of A549 using 15212-1-AP

OGDH Polyclonal antibody

OGDH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

A glycolytic shift in Schwann cells supports injured axons.

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.